Identifying Relapsing Fever Borrelia, Senegal
نویسندگان
چکیده
We describe a nested polymerase chain reaction for the identification of Borrelia species from serum of patients with unidentified fevers. This technique, based on single nucleotide polymorphisms of the 16S ribosomal RNA gene, was used to test blood samples from 7,750 patients, 33 of whom were diagnosed with spirochete infections. Borrelia crocidurae was the only species identified.
منابع مشابه
Tick-Borne Relapsing Fever Borreliosis, Rural Senegal
Detecting spirochetes remains challenging in cases of African tick-borne relapsing fever. Using real-time PCR specific for the 16S rRNA Borrelia gene, we found 27 (13%) of 206 samples from febrile patients in rural Senegal to be positive, whereas thick blood smear examinations conducted at dispensaries identified only 4 (2%) as positive.
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1. Cutler SJ. Possibilities for relapsing fever reemergence. Emerg Infect Dis. 2006;12:369–74. http://dx.doi. org/10.3201/eid1203.050899 2. Bottieau E, Clerinx J, Schrooten W, Van den Enden E, Wouters R, Van Esbroeck M, et al. Etiology and outcome of fever after a stay in the tropics. Arch Intern Med. 2006;166:1642–8. http://dx.doi. org/10.1001/archinte.166.15.1642 3. Vial L, Diatta G, Tall A, ...
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BACKGROUND The ongoing drought in sub-Saharan countries has led to the colonisation of west African Savanna by Ornithodoros sonrai; this tick acts as a vector for Borrelia crocidurae, which causes tick-borne relapsing fever (TBRF). Our aim was to ascertain the incidence of TBRF in west Africa. METHODS From 1990 to 2003, we monitored the incidence of TBRF in Dielmo, Senegal, by daily clinical ...
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References 1. Parola P, Diatta G, Socolovschi C, Mediannikov O, Tall A, Bassene H, et al. Tick-borne relapsing fever borreliosis, rural Senegal. Emerg Infect Dis. 2011;17:883–5. 2. Elbir H, Raoult D, Drancourt M. Relapsing fever borreliae in Africa. Am J Trop Med Hyg. 2013;89:288–92. http://dx.doi.org/ 10.4269/ajtmh.12-0691 3. Wilting KR, Stienstra Y, Sinha B, Braks M, Cornish D, Grundmann H. L...
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The quantitative buffy coat (QBC) parasite detection method is a sensitive and specific tool for the diagnosis of malaria parasites. It is also useful for the diagnoses of other hemoparasites, including Trypanosoma, Babesia, and Leptospira. We report a case of relapsing fever diagnosed by this technique in a short-term traveler from Senegal. The diagnosis was confirmed by the standard Giemsa he...
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